Serum lipids and lipoproteins were measured in PHINOX and PDHS

Tissue culture plasticware was purchased from Corning (Corning, NY) and Thermo Fisher (Rochester, NY). RPMI 1640 and phosphate-buffered saline (PBS) were purchased from Mediatech Cellgro (Manassas, VA). MEM buffered with 10mM Hepes (pH 7.4) was prepared in the laboratory from MEM purchased from BioWhitaker (Walkersville, MD) and Hepes purchased from Fisher Scientific (Newark, DE). Fetal bovine serum (FBS), 8-(parachlorophenylthio) cyclic-3’,5’-amp (CTP-cAMP, cAMP), calf serum (CS), gentamicin, trypsin EDTA, polyethylene glycol (PEG) 6000, Probucol and the ACAT inhibitor Sandoz 58-035 were all purchased from Sigma-Aldrich (St. Louis, MO). Organic solvents were purchased from VWR (Radnor, PA). BSA was purchased from Millipore (Kankakee, IL) and [1,2-H]-cholesterol was purchased from Perkin Elmer Analytical Sciences (Waltham, MA).